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Old 07-21-2011, 08:57 AM   #1
Vern
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Default miRNA library prep

I am having trouble size selecting on the TBE gels for the sm RNA preps? Any advice on what dyes and stains work best?
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Old 07-25-2011, 03:10 PM   #2
advanT
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Default staining TBE gel

We use SYBR for staining miRNA libraries post PCR. Our buffers gel loading buffers come from a kit. Resolution and separation of miRNAs products works well with SYBR.
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Old 03-21-2012, 10:57 AM   #3
shobbir
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what percentage gel works best? did you use a TBE-Urea gel?
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Old 03-23-2012, 01:45 PM   #4
NextGenSeq
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8% no urea
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Old 03-23-2012, 02:00 PM   #5
shobbir
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i was under the impression it is standard to use denaturing conditions when running RNA?
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Old 03-26-2012, 10:33 AM   #6
cascoamarillo
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Hi
Not sure what are you asking for...isolating small RNAs or libraries made from miRNAs?
At least, I did it (small RNA isolation) with 15% denaturing (UreaGel from National Diagnostics) PAGE.
The library purification (eliminate any kind of primer dimer, adapters, etc) I used 10% non-denatuting PAGE.
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