Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Rapid screening of complex DNA samples by single-molecule amplification and s Newsbot! Literature Watch 0 06-01-2011 03:00 AM
PubMed: A novel single cDNA amplicon pyrosequencing method for high-throughput, cost- Newsbot! Literature Watch 0 07-24-2010 03:00 AM
PubMed: Bind-n-Seq: high-throughput analysis of in vitro protein-DNA interactions usi Newsbot! Literature Watch 0 10-22-2009 03:00 AM
PubMed: Genome-wide high throughput analysis of DNA methylation in eukaryotes. Newsbot! Literature Watch 0 10-28-2008 06:00 AM

Thread Tools
Old 04-16-2008, 08:51 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered

Syndicated from PubMed RSS Feeds

Related Articles High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets.
Anal Chem. 2008 Apr 15;
Authors: Kumaresan P, Yang CJ, Cronier SA, Blazej RG, Mathies RA
A high-throughput single copy genetic amplification (SCGA) process is developed that utilizes a microfabricated droplet generator (microDG) to rapidly encapsulate individual DNA molecules or cells together with primer functionalized microbeads in uniform PCR mix droplets. The nanoliter volume droplets uniquely enable quantitative high-yield amplification of DNA targets suitable for long-range sequencing and genetic analysis. A hybrid glass-polydimethylsiloxane (PDMS) microdevice assembly is used to integrate a micropump into the microDG that provides uniform droplet size, controlled generation frequency, and effective bead incorporation. After bulk PCR amplification, the droplets are lysed and the beads are recovered and rapidly analyzed via flow cytometry. DNA targets ranging in size from 380 to 1139 bp at single molecule concentrations are quantitatively amplified using SCGA. Long-range sequencing results from beads each carrying approximately 100 amol of a 624 bp product demonstrate that these amplicons are competent for achieving attomole-scale Sanger sequencing from a single bead and for advancing pyrosequencing read-lengths. Successful single cell analysis of the glyceraldehyde 3 phosphate dehydrogenase (GAPDH) gene in human lymphocyte cells and of the gyr B gene in bacterial Escherichia coli K12 cells establishes that SCGA will also be valuable for performing high-throughput genetic analysis on single cells.
PMID: 18410131 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:16 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO