Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • cuffdiff- no assembled transcripts

    Hi

    We are running tophat pipeline on algal sequences and our cuffdiff output does not appear to have assembled transcripts listed, rather CDS that correlate w/ single exons only.

    tophat:
    tophat2 -o ./psr1_rep_s2 -i 50 -I 28000 --min-segment-intron 50 --max-segment-intron 12800 -G Creinhardtii_236_gene_exons.gtf $BOWTIE_INDEX/bowtie2/cre5.3 psr1_p_replete_s2.fastqsanger.gz

    cufflinks:
    cufflinks -o ./cufflinks_out_psr1_rep_s2 -I 50000 -N –g Creinhardtii_236_gene_exons.gtf tophat_out_psr1_rep_s2/accepted_hits.bam

    cuffdiff:
    cuffdiff -o ./cuffdiff_out -L 1690_rep,psr1_rep,1690_dep,psr1_dep /merged_asm/merged.gtf list of accepted_hits.bam


    example cuffdiff which returns tests for DE for each exon, but not entire transcript:
    XLOC_000003 XLOC_000003 PAC:27578295.CDS.1 chromosome_1:20355-20687
    XLOC_000004 XLOC_000004 PAC:27578295.CDS.2 chromosome_1:20759-21065
    XLOC_000005 XLOC_000005 PAC:27578295.CDS.3 chromosome_1:21137-21443
    XLOC_000006 XLOC_000006 PAC:27578295.CDS.4 chromosome_1:21513-21819
    XLOC_000007 XLOC_000007 PAC:27578295.CDS.5 chromosome_1:21986-22132
    XLOC_000008 XLOC_000008 PAC:27578295.CDS.6 chromosome_1:22453-22699
    XLOC_000009 XLOC_000009 PAC:27578295.exon.7 chromosome_1:22862-22948
    XLOC_000010 XLOC_000010 PAC:27578295.exon.8 chromosome_1:23106-23250
    XLOC_000011 XLOC_000011 PAC:27578295.exon.9 chromosome_1:23370-23957


    What am I missing besides the obvious?

    thanks

    Charles

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
59 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
57 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
51 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X