ThruPLEX kit: anyone who has some experience?

Simone78 · 01-02-2015, 03:24 AM

Dear all,

I was trying to figure out what the kit ThruPLEX contains.
In the US Patent 8,206,913 there are a lot of interesting info about the more recent PicoPLEX kit, so one can find out exactly what each tube contains.

However, for the ThruPLEX kit (US Patents 7,803,550-8,071,312-8,399,199) the protocols and PCR programs reported in the patent are completely different from the manual you get with the kit.
How the hairpin oligos work and how they don´t form dimers seems really clear...but I´m deeply confused about the rest!

Anybody out there who tried to figure out what he/she is doing when adding reagents from all those colourful tubes?

Thanks!

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01-02-2015, 03:24 AM	#1
Simone78 Senior Member Location: Basel (Switzerland) Join Date: Oct 2010 Posts: 208	ThruPLEX kit: anyone who has some experience? Dear all, I was trying to figure out what the kit ThruPLEX contains. In the US Patent 8,206,913 there are a lot of interesting info about the more recent PicoPLEX kit, so one can find out exactly what each tube contains. However, for the ThruPLEX kit (US Patents 7,803,550-8,071,312-8,399,199) the protocols and PCR programs reported in the patent are completely different from the manual you get with the kit. How the hairpin oligos work and how they don´t form dimers seems really clear...but I´m deeply confused about the rest! Anybody out there who tried to figure out what he/she is doing when adding reagents from all those colourful tubes? Thanks!