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05-11-2017, 02:28 PM
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#1 |
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Junior Member
Location: Chicago Join Date: Mar 2017
Posts: 6
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UMI in Smart-seq2 protocol
Hi All,
Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account of anyone doing so. I would like to know if it is even possible. Thank you! |
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05-11-2017, 04:10 PM
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#2 |
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Jafar Jabbari
Location: Melbourne Join Date: Jan 2013
Posts: 1,238
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For this protocol UMI can be added to RT primer and SMART oligo. But they will be lost once amplified cDNA is fragmented/tagmented. With some modifications it is possible to keep primer and SMART oligo UMIs but reads from main body of transcript (except termini) will not have UMI.
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| Tags |
| cdna synthesis, rna-seq, smart-seq2, umi |
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