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01-17-2011, 11:48 AM
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#1 |
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Junior Member
Location: Champaign Illinois Join Date: Jan 2011
Posts: 1
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custom primer
I know only a little about Illumina's sequencing. Is it possible to use custom primers in Illumina's flow channel? Anybody know how their primer is attached to the polyacrylamide coating?
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01-18-2011, 11:07 AM
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#2 |
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Member
Location: San Francisco, CA Join Date: Jun 2010
Posts: 20
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It isn't too hard. It's best to just make your oligos as similar as possible to Illumina's own. You should probably refer to this earlier thread on how to design the primers and adapters:
http://seqanswers.com/forums/showthread.php?t=198 |
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01-18-2011, 01:46 PM
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#3 |
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Senior Member
Location: Bethesda MD Join Date: Oct 2009
Posts: 509
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I think the question is whether the oligos attached to the flow cell can be customized. Unfortunately, that is not possible.
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01-18-2011, 02:01 PM
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#4 |
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Member
Location: San Francisco, CA Join Date: Jun 2010
Posts: 20
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Flowcell
I looked at Illumina's method and it seems like a big pain, especially if you're not Illumina. I bet Illumina wouldn't like it either. Let us know if you're ever successful.
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