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Does anyone have any advice on denoising Illumina reads (specifically 16s reads from a HiSeq)? I've had a look in the literature and most of the denoising algorithms available seem to be aimed at 454 sequencing (and not really applicable to Illumina since they work on flowgram files). The only real possibility seems to be Rosen et al (2012). I would be very interested to hear about anyone's experience using this or any other approach for denoising Illumina data.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM