SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
opinions on rna-seq validation nsl RNA Sequencing 2 08-05-2013 10:55 PM
experiences ncPRO-seq Dolphin22 RNA Sequencing 1 05-08-2013 10:22 AM
Best RNA-Seq Analytical Pipeline to use - opinions ? gendxdoc RNA Sequencing 2 08-14-2012 05:19 AM

Reply
 
Thread Tools
Old 06-01-2015, 07:06 AM   #1
acornwell
Junior Member
 
Location: Upstate NY

Join Date: Feb 2013
Posts: 2
Default Opinions/Experiences with targeted RNA-Seq?

Hello,
I recently had a people ask me about possible options for cheaper sequencing for when there are pre-defined genes of interest, and I started to look into targeted RNA-Seq, specifically from Illumina since our core facility has a MiSeq and it would be great for that. We sometimes order TLDA PCR arrays for validation, but that is relatively expensive for larger numbers of samples. With the targeted system, we could pick 100-ish genes and then just multiplex to run all samples in one lane, in which case the library kit/probe order would be the most expensive part.

I saw a couple of threads on here about targeted RNA-Seq but not many responses. Does anyone have any experience or opinions on it compared to running RT-qPCR on TLDAs or OpenArrays? Is it less expensive (through Illumina) than TLDAs for 10-20 samples and 50 - 100 genes?

Thanks!
acornwell is offline   Reply With Quote
Reply

Tags
illumina, targeted rna-seq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:57 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO