Hi I'm trying to bring RNA-seq into our lab. Our samples will be variable +/-1000 cells, 3-4000 cells total. After I lyse the cells for RNA, should I run the entire sample or should I get the concentration of RNA and use a specific total RNA amount for making each library? How do you adjust for between sample variability and for that matter within sample variability? Or does this come out in the wash during normalization of the data?
Thank you.
Thank you.
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