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I have what is probably a stupid question, but I'm really not sure what to do. How do people usually use distance to TSS when designing primers for ChIP-qPCR validation? I was planning on using UCSC genome browser, but I'm not sure whether distance to TSS factors in the 5'UTR exon when trying to pull the sequence for a gene. For example, one of the genes that came up in a ChIP-Seq I did was Nhe1, and the distance to TSS is -17. Is that -17 with respect to the atg (which would include the 5'utr sequence), or is it -17 in the 'Promoter/Upstream' sequence that you get if you check the box in the genomic sequence? They aren't the same for most of the genes that I've checked so far. Any help would be greatly appreciated.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM