Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    Originally posted by id0 View Post
    You can try trimming your FASTQ files and see how that goes. Sickle trims based on quality scores, so you would only lose low quality bases:
    https://github.com/najoshi/sickle
    We'll give that a try. Thanks!

    Comment


    • #17
      Originally posted by id0 View Post
      My original BWA issue did not get resolved. I am using Mac OS X 10.7 and I have seen reports of other Mac users reporting the same problem. When I switched from BWA 0.6.2 to BWA 0.5.9, it was working fine.

      As I was writing this reply, I noticed BWA 0.7.0 was released last week. I am not sure if that one solves any of the Mac issues. See changelog here:
      Burrow-Wheeler Aligner for short-read alignment (see minimap2 for long-read alignment) - File not found · lh3/bwa




      I used BWA for 2x250bp reads and I did not encounter any problems. From BWA main page:
      hi,
      It looks like the last 3 versions of bwa, including the latest released last week, don't work on a Mac. I downloaded 0.5.10 and I think things are working now. It's definitely taking much longer to make the .sai file, so I see that as a good sign.
      Thanks!

      Comment


      • #18
        I just ran into this after I aligned human paired-end sequences against the tiny non-human genome included in the distribution kit.
        Opps:-(
        Bill

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Essential Discoveries and Tools in Epitranscriptomics
          by seqadmin




          The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
          04-22-2024, 07:01 AM
        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 08:47 AM
        0 responses
        12 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        60 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        59 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        54 views
        0 likes
        Last Post seqadmin  
        Working...
        X