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Old 04-21-2014, 06:42 AM   #1
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Location: Massachusetts

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Default RNA-seq analysis with stranded and unstranded data


I have a dataset in which 30 samples are strand specific and 6 are unstranded. Is there any way to analyze these samples together for gene expression? Both sets were sequenced using Illumina Tru Seq. Would greatly appreciate any help.


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Old 04-21-2014, 08:09 AM   #2
Devon Ryan
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Just include the library-type as a factor in the design.
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Old 04-30-2014, 03:58 AM   #3
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You could try library-type to determine the pipeline design
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