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Old 06-28-2010, 03:43 AM   #1
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Location: London

Join Date: Jun 2009
Posts: 5
Default Understanding Maq log output


I have a couple of questions about my Maq map output if anyone knows the answers I'd really appreciate them:

[match_search]  98% processed in 20717.101 sec: 31618863 / 21294334 = 1.485
[match_search]  99% processed in 20765.187 sec: 28388678 / 20109661 = 1.412
[match_search] 100% processed in 20768.725 sec: 1164880 / 1048335 = 1.111
[match_core] sorting the hits and dumping the results...
[ma_load_reads] loading reads...
[ma_load_reads] 2000000*2 reads loaded.
[mapping_count_single] 7, 11, 16, 27
[maq_indel_pe] the indel detector only works with short-insert mate-pair reads.
[match_data2mapping] 1922258 out of 4000000 raw reads are mapped with 0 in pairs.
-- (total, isPE, mapped, paired) = (2000000, 0, 1922258, 0)
First, what is the "mapping_count_single" - what do these 4 numbers mean?

Second, this file contained 2,000,000 reads (8 million lines in fastq format), so how come the logfile says x out of 4000000 raw reads are mapped?

Many Thanks
anna_vt is offline   Reply With Quote
Old 10-17-2011, 07:48 PM   #2
Location: Sydney

Join Date: Aug 2011
Posts: 12

I have a similar issue. My output log reports n_reads*2 because they are paired end, but it looks like that is not the case for your data. My issue is the "x out of n raw reads are mapped" part:

[match_data2mapping] 1976956 out of 2000000 raw reads are mapped with 1969400 in pairs.
-- (total, isPE, mapped, paired) = (2000000, 1, 1976956, 1969400)

What happened to the remaining 23,044 reads? It is not that they are simply unmapped, as I have a number of unmapped reads in my .sam alignment file.

Any help much appreciated.
Tally is offline   Reply With Quote

maq map, output

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