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Old 04-05-2018, 07:51 AM   #1
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Default Prevention of index hopping in Illumina tech

Illumina has announced free adapter blocking agents which are supposed to help minimize index hopping.
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Old 04-05-2018, 10:43 AM   #2
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Not clear what this is. Or what "minimizing index hopping" means. What fold reduction are they seeing with use of this reagent?

More info, including the manual here:

As always, I'm less worried about "free adapter" per se. It's the adapter strands annealed to full length library molecules that tend to escape clean-up.

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Old 08-24-2020, 03:20 AM   #3
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Anyone know if the Free Adapter Blocking Reagent is compatible with two-step PCR amplicon libraries? Anyone know how the reagent works?
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illumina, index hopping

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