Primer3 issue

[titaji]

Dear all

I'm new here and I have a problem with Primer3 program.

I'm using MISA and Primer3 in pipeline. After I got *.misa file, I put this syntax.

p3_in.pl FASTA.fa.misa

Then I got empty file of *.p3in. Is it normal?

By the way, I still tried to run further syntax.

primer3_core < FASTA.fa.p3in > FASTA.fa.p3out

This one showed me the description and usage of Primer3.

I put further syntax which is...

p3_out.pl FASTA.fa.p3out FASTA.fa.misa

It showed the error message like this.

Use of uninitialized value $count in concatenation (.) or string at C:\Users\admin\Documents\Work\NGS_results\MISA\p3_out.pl line 76.

Primer modelling was successful for sequences.
Use of uninitialized value $count_failed in concatenation (.) or string at C:\Users\admin\Documents\Work\NGS_results\MISA\p3_out.pl line 77.
Primer modelling failed for sequences.

Could anyone please help me to solve this issue?

Thank you,
titaji