Hello everyone!!
This is my first post here and I hope it is in right section...
I have to isolate RNA from tissue samples and purpose is to perform microRNA profiling via next generation sequencing. As the first step is RNA isolation and I have Ambion mirvana kit for this. The kit has two protocols, one is Total RNA isolation and other is small RNA enrichment. I would like to know that for miRNA profiling should I follow protocol of Total RNA isolation or the small RNA enrichment protocol?
Regards
Azeem
This is my first post here and I hope it is in right section...
I have to isolate RNA from tissue samples and purpose is to perform microRNA profiling via next generation sequencing. As the first step is RNA isolation and I have Ambion mirvana kit for this. The kit has two protocols, one is Total RNA isolation and other is small RNA enrichment. I would like to know that for miRNA profiling should I follow protocol of Total RNA isolation or the small RNA enrichment protocol?
Regards
Azeem
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