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Old 06-02-2016, 01:59 PM   #1
lguzmanz
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Default CANU-correct subreads individually VS batch

When my Pacbio sequencing was done I ended up with 18 subreads that each contained xxxx number of sequences (6,431,149 sequences in total for all 18 subreads). Due to particular reasons of having an allocation at a super computer I was having troubles running the Canu command that encompasses all three stages: correct, trim, assemble. So what I decided to do was individually execute the Canu-correct command on each of the 18 subreads. So now I have a list of 18 subreads that have been corrected. My questions is would there be a difference if I would have executed the Canu-correct command on one single file that was a concatenation of all 18 subreads? I ask because I am going to do some downstream analysis with the corrected reads and want to make sure that I am doing this right. Thank you!
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Old 06-05-2016, 11:43 AM   #2
gconcepcion
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For consistency - this was answered here:
https://github.com/marbl/canu/issues/149
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Old 06-08-2016, 08:38 AM   #3
lguzmanz
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Thank you @gconcepcion! I will do the same for any further posts. Cheers!
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