Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Pippin Prep vs. gel elution for RNA-Seq shurjo Sample Prep / Library Generation 2 07-04-2013 03:30 PM
Good Beads / Best Beads paolo.kunder SOLiD 2 03-27-2013 02:12 PM
P1 beads quark SOLiD 2 08-15-2012 07:41 PM
Calibration beads mestro2 Sample Prep / Library Generation 1 11-30-2010 07:59 AM
Elution volume after A-tailing? greigite Illumina/Solexa 2 11-01-2009 01:45 PM

Thread Tools
Old 07-03-2012, 09:53 AM   #1
Location: Pittsburgh

Join Date: Aug 2011
Posts: 72
Default elution from streptaviden beads

I was wondering what conditions give the best elution efficiencies from dynal magnetic streptaviden beads. According to the invitrogen (, the best buffers seem to include 95% formamide. However, I would like to purify with Ampure after the elution. I contacted Beckman Coulter and they said that formamide removal wasn't tested. The protocol I am using now has 3 min incubation at 95oC in water to break the streptaviden-biotin bond. Does anyone have any recommendations from their experience with these beads?
shawpa is offline   Reply With Quote
Old 07-09-2012, 12:17 AM   #2
Location: Paris - France

Join Date: Jan 2012
Posts: 13

If the biotin is only on one strand of your library, just try to denature your DNA fragments, it will be easier than breaking Streptavidin-biotin bond (Heat or Melt solution).

To infinity ... and behond !!!
kant1_IC is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:43 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO