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Old 07-03-2012, 09:53 AM   #1
shawpa
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Default elution from streptaviden beads

I was wondering what conditions give the best elution efficiencies from dynal magnetic streptaviden beads. According to the invitrogen (http://www.invitrogen.com/site/us/en...eptavidin.html), the best buffers seem to include 95% formamide. However, I would like to purify with Ampure after the elution. I contacted Beckman Coulter and they said that formamide removal wasn't tested. The protocol I am using now has 3 min incubation at 95oC in water to break the streptaviden-biotin bond. Does anyone have any recommendations from their experience with these beads?
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Old 07-09-2012, 12:17 AM   #2
kant1_IC
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Default

If the biotin is only on one strand of your library, just try to denature your DNA fragments, it will be easier than breaking Streptavidin-biotin bond (Heat or Melt solution).

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