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  • Ion Proton Bead Size

    Is anyone aware of any literture outlining the bead size used in the Ion Proton sequencing platform?

    I have seen a lot of off-hand accounts that they are ~300 nm, however I have yet to see anything directly confirming this in the literature or from Ion Torrent.

  • #2
    I seem to recall seeing somewhere that the wells on the P1 chip are ~1uM and the beads ~0.6uM. Someone posted some EM pictures on Seqanswers a while back but I can no longer find the post.

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    • #3
      Originally posted by mbzmg1 View Post
      I seem to recall seeing somewhere that the wells on the P1 chip are ~1uM and the beads ~0.6uM. Someone posted some EM pictures on Seqanswers a while back but I can no longer find the post.
      Thanks. I just did a search and was able to find the post.
      Integrated electronic detection of protons released upon base incorporation. (Life Technologies)


      There aren't any images of the Proton beads, but for the size of the wells ~600 nm does seem to be appropriate.

      I'll keep searching and post here if I find out anything new.

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