Hi there,
I have some basic questions. It would be really great if any one could help me out.
1. I wanted to know different ways of demultiplexing. I have worked with demultiplex.pl script targeting barcodes.
Is there any scripts or ways targeting sequence.
I want both the F and R reads in one file the one i am using gives me seperate file (demultiplex.pl).
2. After demultiplexing i get different number of reads in F and R files (i suppose it to be equal in number). What would be the threshold of difference in the number of reads in F and R files?
Thanks
I have some basic questions. It would be really great if any one could help me out.
1. I wanted to know different ways of demultiplexing. I have worked with demultiplex.pl script targeting barcodes.
Is there any scripts or ways targeting sequence.
I want both the F and R reads in one file the one i am using gives me seperate file (demultiplex.pl).
2. After demultiplexing i get different number of reads in F and R files (i suppose it to be equal in number). What would be the threshold of difference in the number of reads in F and R files?
Thanks
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