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Old 12-09-2015, 08:38 AM   #21
Location: United States

Join Date: May 2014
Posts: 40

Definitely a good idea to store at a higher concentration than needed for submission. For reference, you'll need to redilute your 151nM library to 10nM with e.g. 5uL of your library in 70.5uL of dilution buffer or whatever volumes work best for you. Illumina recommends Tris-Cl 10*mM, pH*8.5 with 0.1% Tween 20 rather than water.
adam.geber is offline   Reply With Quote
Old 12-09-2015, 09:54 AM   #22
Location: bUNDOORA

Join Date: Apr 2015
Posts: 18

Thanks adam.geber. I don't know but I was asked to send samples in MQ water.
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