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Old 08-02-2011, 03:17 PM   #1
Location: Boston, MA

Join Date: Oct 2009
Posts: 15
Default Sequencing ChIPseq samples prepped with micrococcal nuclease?

I was wondering if anyone has had any issues sequencing samples that were prepped using a micrococcal nuclease?

I ran a flow cell that contained mostly these types of samples and the T channel was off the charts during the 1st few bases which basically made it look like everything was over clustered; even though I was running these samples at 10pM, which is my norm.

Does anyone have any experience sequencing these types of samples on the Illumina GAIIx?

cmawhinney is offline   Reply With Quote

chipseq, gaiix, illumina

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