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Old 08-02-2011, 03:17 PM   #1
cmawhinney
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Location: Boston, MA

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Default Sequencing ChIPseq samples prepped with micrococcal nuclease?

I was wondering if anyone has had any issues sequencing samples that were prepped using a micrococcal nuclease?

I ran a flow cell that contained mostly these types of samples and the T channel was off the charts during the 1st few bases which basically made it look like everything was over clustered; even though I was running these samples at 10pM, which is my norm.

Does anyone have any experience sequencing these types of samples on the Illumina GAIIx?

Thanks!
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