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Old 02-28-2012, 03:06 AM   #1
Location: Vienna

Join Date: Mar 2008
Posts: 45
Default Bioanalyzer peak at 107bp

we're getting a narrow single peak at 107bp after size selection on gel and subsequent PCR.
Apart from that, bioanalyzer profiles of the libraries look fine.
I'm pretty sure there have been already some threads about this issue but I couldn't find them using the search function.
As adapter dimers were already removed at the size selection step it must be a product that forms during PCR.
Can someone confirm my theory or even better, tell me how to get rid of this peak?
We're using a ChIP-seq protocol starting with 5-15ng Material.
andibody is offline   Reply With Quote
Old 02-28-2012, 09:16 AM   #2
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Location: St. Louis

Join Date: Dec 2010
Posts: 535

You probably are not eliminating all of the adapter dimer.
Heisman is offline   Reply With Quote

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