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Old 02-28-2012, 03:06 AM   #1
andibody
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Location: Vienna

Join Date: Mar 2008
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Default Bioanalyzer peak at 107bp

Hi,
we're getting a narrow single peak at 107bp after size selection on gel and subsequent PCR.
Apart from that, bioanalyzer profiles of the libraries look fine.
I'm pretty sure there have been already some threads about this issue but I couldn't find them using the search function.
As adapter dimers were already removed at the size selection step it must be a product that forms during PCR.
Can someone confirm my theory or even better, tell me how to get rid of this peak?
We're using a ChIP-seq protocol starting with 5-15ng Material.
Thanks
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Old 02-28-2012, 09:16 AM   #2
Heisman
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Location: St. Louis

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You probably are not eliminating all of the adapter dimer.
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