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Old 06-19-2012, 11:41 AM   #1
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Location: Laramie, WY

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Default Sequencing through high GC content repeat region

I've sequenced a viral genome with the Illumina HiSeq but have some holes where there are repeat regions. The whole genome is 73% GC-rich so the repeats make it even harder to get through. I want to re-sequence these areas, does anyone have any suggestions or methods that helps get accurate sequence data from repeats with high GC content?
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