Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
AMPureXP for ssDNA biochembug Sample Prep / Library Generation 7 05-11-2012 06:40 AM
ssDNA in sample from targeted exon capture affecting quantification vasvale Sample Prep / Library Generation 3 08-08-2011 03:43 AM
Loading a flow cell with ssDNA (one strand only) Yepler Illumina/Solexa 5 05-23-2011 12:37 PM
ssDNA - what happens during sequencing? Kerensa Sample Prep / Library Generation 2 03-17-2011 02:23 PM
Heat shock ssDNA generation-Illumina JBuenrostro Sample Prep / Library Generation 0 03-04-2010 01:32 PM

Thread Tools
Old 03-07-2013, 12:27 PM   #1
Junior Member
Location: Boston

Join Date: Mar 2013
Posts: 1
Default Shearing ssDNA

I'm trying to prepare RNA samples for 3' end mapping, and would like to shear them at the ssDNA stage after first strand synthesis. Does anyone know of a method to reliably shear ssDNA into suitably sized fragments for RNAseq?
aschiff is offline   Reply With Quote

library generation, shear, ssdna

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:44 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO