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Old 03-07-2013, 12:27 PM   #1
aschiff
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Location: Boston

Join Date: Mar 2013
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Default Shearing ssDNA

I'm trying to prepare RNA samples for 3' end mapping, and would like to shear them at the ssDNA stage after first strand synthesis. Does anyone know of a method to reliably shear ssDNA into suitably sized fragments for RNAseq?
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