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Help with RSEM: how to change default phred33 to phred64 LearningBIOINFO RNA Sequencing 7 03-19-2014 10:21 PM

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Old 11-22-2019, 12:17 PM   #1
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Location: Netherlands

Join Date: Nov 2019
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Default Phred33

Hi everyone!

I 've been searching for long time why subtract 33 in :


but I cannot find the answer, somebody could please share a link, give some information where to find the answer or teach me why?

Many thanks in advance!!
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Old 11-23-2019, 07:35 AM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

If you want to represent the Phred score as an text character, then you need to use "letters". Look at the list of ASCII characters and what the first 32 represent

You don't want a quality score of 10 to be represented by a line feed, for example!
Providing nextRAD genotyping and PacBio sequencing services.
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