Hi All,
Recently got bacterial genome assembled using Velvet. Reads were generated from 454. The assembly got quite a bit of runs of "Ns". I thought, 454s does not generate a N. AM wondering what could be the source of these Ns.
Possibly, velvet assigns them when the 454 scores are not great in the overlapping regions of the reads? Any pointers?
Thanks very much community.
Gowthaman
Recently got bacterial genome assembled using Velvet. Reads were generated from 454. The assembly got quite a bit of runs of "Ns". I thought, 454s does not generate a N. AM wondering what could be the source of these Ns.
Possibly, velvet assigns them when the 454 scores are not great in the overlapping regions of the reads? Any pointers?
Thanks very much community.
Gowthaman
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