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Old 12-15-2010, 04:42 PM   #1
Location: China beijing

Join Date: Jan 2010
Posts: 15
Default One question about Tophat

When I use Tophat to get the unqiue reads which just mapped to one location in the genome, I have any problems. When I get the sam file, I filter it with the criterion that the last column is "NH:i:1", but these reads also have more than one location in the genome. I want to know how to reslove this question. Thanks for your attention.
fgh1124 is offline   Reply With Quote

bioinformatics, mapping, rna-seq, tophat

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