SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing
Similar Threads
Thread Thread Starter Forum Replies Last Post
General question about TopHat mhayes Bioinformatics 1 02-03-2012 02:00 PM
question about tophat camelbbs Bioinformatics 7 07-07-2011 06:24 PM
a question about tophat and cufflinks camelbbs Bioinformatics 0 06-27-2011 09:21 AM
Simple Tophat question kwatts59 RNA Sequencing 2 06-02-2011 07:31 PM
a question about tophat lei RNA Sequencing 12 03-20-2011 12:53 PM

Reply
 
Thread Tools
Old 12-15-2010, 04:42 PM   #1
fgh1124
Member
 
Location: China beijing

Join Date: Jan 2010
Posts: 15
Default One question about Tophat

When I use Tophat to get the unqiue reads which just mapped to one location in the genome, I have any problems. When I get the sam file, I filter it with the criterion that the last column is "NH:i:1", but these reads also have more than one location in the genome. I want to know how to reslove this question. Thanks for your attention.
fgh1124 is offline   Reply With Quote
Reply

Tags
bioinformatics, mapping, rna-seq, tophat

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off



All times are GMT -8. The time now is 10:22 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO