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12-15-2010, 04:42 PM
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Member
Location: China beijing Join Date: Jan 2010
Posts: 15
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One question about Tophat
When I use Tophat to get the unqiue reads which just mapped to one location in the genome, I have any problems. When I get the sam file, I filter it with the criterion that the last column is "NH:i:1", but these reads also have more than one location in the genome. I want to know how to reslove this question. Thanks for your attention.
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| Tags |
| bioinformatics, mapping, rna-seq, tophat |
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