Hi,
I am a beginner in high throughput sequencing so I need help with some basic questions about sample preparation (for a bacterial resequencing project on Illumina HiSeq platform, 30x coverage)
1. I have seen somewhere that the usual kits for isolation of genomic DNA are not recommended. Can you tell me why this is the case? If it is about the DNA size, why does this matter if DNA is anyway to be sheared into small pieces for sequencing?
2. What is a library quality control and is it usual to have one?
Thanks!
I am a beginner in high throughput sequencing so I need help with some basic questions about sample preparation (for a bacterial resequencing project on Illumina HiSeq platform, 30x coverage)
1. I have seen somewhere that the usual kits for isolation of genomic DNA are not recommended. Can you tell me why this is the case? If it is about the DNA size, why does this matter if DNA is anyway to be sheared into small pieces for sequencing?
2. What is a library quality control and is it usual to have one?
Thanks!
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