Hi every one,
After I aligned the reads of my samples with tophat, do I need to to create an index for my reference genome like after bowtie2 alignment, and then convert/sort/index all my sam files to identify the upregulated and down-regulated genes from a compound treatment?
Thanks a lot!!
Richard
After I aligned the reads of my samples with tophat, do I need to to create an index for my reference genome like after bowtie2 alignment, and then convert/sort/index all my sam files to identify the upregulated and down-regulated genes from a compound treatment?
Thanks a lot!!
Richard
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