Hello
I observed a strange sequence result for two times.
The run seemed OK until a certain point, and sequnce quality collapse and the low sequence quality retained until the end of the run.
Two Times !!!
I found that the sequnce quality drop down right after resetting new primer. and I found that there was a reset process right before applying new primer from the log file.
I checked the reset reagent, and the reset reagent bottle was almost empty!!!
I am not sure that the low sequence quality mainly from the shortage of the reset reagent, but I am kind of positive.
Anyone has an experience of running out of the reset reagent?
Any idea about the low sequence quality observed from the first cycle of new primer?
I observed a strange sequence result for two times.
The run seemed OK until a certain point, and sequnce quality collapse and the low sequence quality retained until the end of the run.
Two Times !!!
I found that the sequnce quality drop down right after resetting new primer. and I found that there was a reset process right before applying new primer from the log file.
I checked the reset reagent, and the reset reagent bottle was almost empty!!!
I am not sure that the low sequence quality mainly from the shortage of the reset reagent, but I am kind of positive.
Anyone has an experience of running out of the reset reagent?
Any idea about the low sequence quality observed from the first cycle of new primer?
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