Hi,
I got a problem in my library preparations.
I tried to prepare 180/230 bp libraries. I have used NEB reagents and illumina PE adapters and primers for my library preparations.
I am getting the expected size fragments after PCR enrichment. However, the concentration is too low, ~10 ng/ul. I have repeated the same many times but did not get a high yield.
Could anyone please tell me what should I change to get high yield?
Thanks.
I got a problem in my library preparations.
I tried to prepare 180/230 bp libraries. I have used NEB reagents and illumina PE adapters and primers for my library preparations.
I am getting the expected size fragments after PCR enrichment. However, the concentration is too low, ~10 ng/ul. I have repeated the same many times but did not get a high yield.
Could anyone please tell me what should I change to get high yield?
Thanks.
Comment