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  • Anyone know what this peak is on my BA trace?

    This is an RNA pico chip and the well is supposed to be empty.
    Attached Files
    - Gina

  • #2
    If wells before it are overloaded, it will show up in random wells. Kind of like the capillary sequencers-if one cap is high flourescence and the neighboring shows nothing, the other sequence may appear to be in that cap

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    • #3
      Hm. That could be it. It says the previous wells are around 200 pg/uL, so I'm not sure, unless some of that sample didn't even get measured and just went into the empty wells. Would it be bunched together in a peak that is >4000 nt like that?
      - Gina

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      • #4
        It can be. When it goes through the capillaries of the chip, it can let go at any time to come out at any size.

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        • #5
          Ah I see. Thanks!
          - Gina

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