Hi,
I am doing RNA-IP on a RNA-binding protein and have the following question:
What is the best way to elute the protein with the RNA from the beads to preserve RNA quality?
I was thinking of using Glycine followed by Phenol extraction of the RNA.
The RNA is not crosslinked to the protein.
I am doing RNA-IP on a RNA-binding protein and have the following question:
What is the best way to elute the protein with the RNA from the beads to preserve RNA quality?
I was thinking of using Glycine followed by Phenol extraction of the RNA.
The RNA is not crosslinked to the protein.