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Does anyone have experience using Illumina HiSeq v3 reagents after their expiry date? Everything is still stored at -20 in original packaging. Kits in question is SR and PE v3 clustering kits and sequencing kits. If so, how far out have you used them? Be aware, my Illumina products arrived with only 3 months left on the shelf life!
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As long as it was stored properly, I don't see what could have gone bad.... -
I was told by my FAS that the SR kits are more robust to being used past expiry than the PE kits. I guess it has something to do with how the linearisation is achieved.
We have some SR kits that are about 6 months out of date and we're going to put them on in a few weeks to see if we get anything, but I have no info at the moment.Comment
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Highly curious: Did you ever run any of the expired SR cluster kits?Comment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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