Hi folks,
I’m working on multiplexing using barcoded adapters – the non-Illumina version where barcodes are read as the 5’ end of the fragment. From various discussions in these forums it sounds like a number of groups are doing this routinely, and with good success. I would like to pool up to 96 samples in a lane. Would anyone multiplexing at this level be willing to share their barcode sequences?
I am, of course, also considering just designing my own… any suggestions on particular sequence patterns to avoid?
Thanks!
Nathan
I’m working on multiplexing using barcoded adapters – the non-Illumina version where barcodes are read as the 5’ end of the fragment. From various discussions in these forums it sounds like a number of groups are doing this routinely, and with good success. I would like to pool up to 96 samples in a lane. Would anyone multiplexing at this level be willing to share their barcode sequences?
I am, of course, also considering just designing my own… any suggestions on particular sequence patterns to avoid?
Thanks!
Nathan
Comment