Hi,
Does anyone know if the two sequences produced by an Illumina paired-end read show the two sequences as being read from opposite ends or are the reads correspond to just forward reading?
Also, the two sequences *.1_sequence.txt and *.2_sequence.txt output from the paired-end reads are not of the same size (these are fastq formatted files). In my case one file is a GB bigger than the other and both are 30GB+ in size. What I really would like to know is if I split the large file into parts, do I interpret the 1_sequence as being going forward and 2_sequence going reverse? Or do I consider both going in just the same direction (say, 5' to 3')?
Any answers or pointers would be highly appreciated...
TiA,
Nash
Does anyone know if the two sequences produced by an Illumina paired-end read show the two sequences as being read from opposite ends or are the reads correspond to just forward reading?
Also, the two sequences *.1_sequence.txt and *.2_sequence.txt output from the paired-end reads are not of the same size (these are fastq formatted files). In my case one file is a GB bigger than the other and both are 30GB+ in size. What I really would like to know is if I split the large file into parts, do I interpret the 1_sequence as being going forward and 2_sequence going reverse? Or do I consider both going in just the same direction (say, 5' to 3')?
Any answers or pointers would be highly appreciated...
TiA,
Nash
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