Hi
I've seen several posts about extending the lifespan of the Nextera tagmentation kits by using a fraction (1/10ish) of the reagents. However, the only published paper talks about doing this using megabase-sized genomes. As I am doing human genome analysis, I am not sure if this will work for me. Does anybody have experience using small volume Nextera reactions with human genome tagmentation?
Thanks for your help!
I've seen several posts about extending the lifespan of the Nextera tagmentation kits by using a fraction (1/10ish) of the reagents. However, the only published paper talks about doing this using megabase-sized genomes. As I am doing human genome analysis, I am not sure if this will work for me. Does anybody have experience using small volume Nextera reactions with human genome tagmentation?
Thanks for your help!
Comment