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I have several lanes of sequences which I have merged into one BAM file. I then processed this merged BAM file through GATK: recal, dedup, etc. I want create a pileup from the merged BAM file using GenomeAnalysisTK.jar -T Pileup . Is there a way to perform this pileup with the lanes separated? When I run Pileup on the merged BAM file, output exists, but it is unclear from which lane the output is generated.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM