Hi everyone,
i've got 200bp metagenomic data based on V3 region and i'm currently using QIIME to analyze it... but i reach family level by default.
I would like to reach species level if possibile, or at least try it... so i've found in the QIIME forum a modified version of the RDP taxonomic table that allow to assign taxonomy to the OTUs down to species level, but it worked for only 5% of my sequences, wile the remaining 95% stopped at genus or even family level.
I'm not a big expert in metagenomics, can you suggest me other tools that i can try in order to analyze my data down to species level and see if i can reach better results?
i've got 200bp metagenomic data based on V3 region and i'm currently using QIIME to analyze it... but i reach family level by default.
I would like to reach species level if possibile, or at least try it... so i've found in the QIIME forum a modified version of the RDP taxonomic table that allow to assign taxonomy to the OTUs down to species level, but it worked for only 5% of my sequences, wile the remaining 95% stopped at genus or even family level.
I'm not a big expert in metagenomics, can you suggest me other tools that i can try in order to analyze my data down to species level and see if i can reach better results?
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