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Old 08-09-2017, 04:59 AM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Default Low input of primers for pre optimized PCR step

I have created a situation where I only have enough reagents to add approximately 1/5 of the primers recommended (pre optimized by Illumina) for a library amplification step (to each well of a plate). Will the amplification still work? I am going to use lower DNA input so as to not 'dilute out' the primers, there are two different input amounts which can be used, I am opting for the lower.
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amplification, library construction, optimization, pcr, reagents

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