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Old 09-27-2012, 05:53 AM   #1
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Location: China

Join Date: Oct 2010
Posts: 1
Post BSSim: Bisulfite sequencing simulator for next-generation sequencing.

BSSim is implemented in the Python language and run in an operating system-independent manner. It can allow users to mimic various methylation level (total methylation level of cytosines, percentage of cytosines that methylated and methylation level of total methylcytosines) and bisulfite conversion rate in CpG, CHG and CHH context, respectively. It can also simulate genetic variations that are divergent from the reference sequence along with the sequencing error and quality distributions. In the output, both directional/non-directional, various read length, single/paired-end reads and alignment data in the SAM format can be generated. BSSim is a cross-platform BS-seq simulator offers output read datasets not only suitable for Illumina's Solexa, but also for Roche's 454 and Applied Biosystems' SOLiD.

Detail information is at:

Download and installation instructions of them are available at

It will be published soon, thank you for your attention and suggestions.

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bis-seq, bisulfite sequencing, dna methylation, simulation

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