Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
nimblegen capture lid temperature weihuameng Sample Prep / Library Generation 0 07-08-2013 06:39 AM
Kapa Quant Kit-- Roche light cycler sanju0891 454 Pyrosequencing 0 05-07-2012 01:13 AM
emPCR thermal cycler settings suefo 454 Pyrosequencing 7 08-16-2011 01:06 AM
emPCR on thermal cycler jbertsche 454 Pyrosequencing 2 07-07-2011 07:52 AM

Thread Tools
Old 05-30-2018, 06:06 AM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Default Thermal cycler with variable temperature lid?

Does this machine exist:, for example, a machine that can change the lid temperature during the course of the reactions? Something tells me that having the lid set to 75C when my reverse transcription reaction is occurring at 45C is not a good idea. In addition, I've been getting lots of adapter dimers and/or technical sequences/artifacts in library prep which I see on the bioanalyzer during my library constructions. Could not having a heat-changing lid be the reason? I am talking about cross all different sample types/library prep kits/depletions.
seqtechno1 is offline   Reply With Quote
Old 05-30-2018, 08:00 PM   #2
Location: Europe

Join Date: Oct 2016
Posts: 60

Hello seqtechno1,

I'm not aware of such a cycler. But having the lid temperature on 75C seems to me very low. For all kind of reaction we use 105C.

But I'm unsure whether this can lead to your problem, as the higher lid temperature should just prevent vaporescence of your sample.

fin swimmer
finswimmer is offline   Reply With Quote

heated lid, library prep, pcr, reverse transcription, thermal cycler

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:14 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO