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Old 05-12-2020, 02:12 PM   #1
Location: US

Join Date: Jan 2016
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Default Poor DNA prep

Hi. I have some samples from another lab that were prepped with the Qiagen kit. All I have is a limited amount of DNA, & the DNA is just okay quality. I did a clean up and ran them on the Miseq. Some of the sequencing quality is poor. Any recommendations for improving the data quality on the analysis side? Or even something to do if I redo the library prep? Thanks! The samples I prepped ran fine, so it isn’t a run issue.
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Old 05-13-2020, 12:27 PM   #2
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Could you let us know what specific problems you see with the data (and the DNA samples?)?
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Old 05-14-2020, 06:05 AM   #3
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What library prep tools are at your disposal? I'd suggest Nextera XT for low-concentration samples. You could also reamplify your libraries prior to normalization. If your samples are low on concentration, try vacuum concentration.
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bioinformatic analaysis, miseq, nextera flex

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