Tweet
Anyone sequencing mate pair libraries beyond the recommended 36bp?
Thanks,
Phillip
Thanks,
Phillip
-
-
I was thinking about doing the same thing as well, has anyone tried this ?Originally posted by PNG View Post
Thanks -
Mate pair length
I recently did a 101-bp run with indexed mate pairs. Rather than cut out a 300-400 bp chunk at the second fragmentation step, I took a 600 bp chunk. So far, we have not seen any odd bioinformatic results indicating a junction-point issue. That said, you can always just trim the ends if it is of concern. I indexed the mate pair and had it in with 4 other bugs that I prepared as paired end. The mate-pair sample definitely was the cleanest of the group, i.e. better quality scores toward the end of the reads.Comment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment