Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • illumina bcl2fastq remove adaptor

    I am running bcl2fastq 2.14. I do not find the parameters about remove adaptors.
    The default will remove adaptors. If I do not want to remove adaptors, what can I do?
    Thank you for help!!

  • #2
    This seems to be the case for bcl2fastq v. 2.15.0.4 also (based on some other posts here, you should upgrade to this version).

    We have not worked with NextSeq data as yet but this is a feature that should be present.

    Comment


    • #3
      Originally posted by mot View Post
      I am running bcl2fastq 2.14. I do not find the parameters about remove adaptors.
      The default will remove adaptors. If I do not want to remove adaptors, what can I do?
      Thank you for help!!
      The bcl2fastq need a sample sheet as input. At the third section called '[Settings]'. You can add your own adapter to it, then bcl2fastq will read the adapter from sample sheet to trim adapter in the reads.

      If you don't want to remove adapters, just do not add your adapter sequence to the sample sheet.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      67 views
      0 likes
      Last Post seqadmin  
      Working...
      X