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Old 03-30-2017, 08:10 AM   #1
renuka22
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Location: US

Join Date: Mar 2017
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Default polyA library preparation and RIN values

Hi there,

I am new to this forum and had some questions about the RNA bioanayzer data and nanodrop readings. I got a good concentration of 200-300ng/ul when I analysed my samples by nanodrop whereas on the bioanalyzer, the compay was not able to obtain any RIN value (Nobands obtained, not even a smear, only some clear area seen on the gel). They concluded saying all the RNA is degraded. Also, they say they are not able to make polyA library as the concentration after polyA shows only around 0.1-0.2 ng/ul and it is very low to get any sequencing results. Anyone can suggest something regarding this, coming from a totally inexperienced person..

Thanks
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Old 03-30-2017, 02:22 PM   #2
nucacidhunter
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Default

Nanodrop QC is only good to detect contaminants not for quantification. RNA quantification can be done using Qubit or similar method and integrity by gel or Bioanalyzer-like instruments. Quantification by Bioanalyzer is Ok for most application though it is not very accurate. I tend to agree with your service provider.
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