Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Ngs...roche 454

    hello everyone
    i am completely new to NGS analysis. I want to analyse roche 454 data which i have downloaded from ena database (in fastq format) for its quality. I have read that roche data is in *fna and *.qual format. So how do i convert this fast q data into *.fna and *.qual format................
    thank you

  • #2
    In the fastq files, the second line of each entry is the read sequence, and the fourth line gives the base qualities.

    Comment


    • #3
      hiiiii......
      if we want to convert the sra data into *.fna and *.qual format......which tool/protocol should we use......?

      Comment


      • #4
        A simple Google search will give you several tools to do the conversion you want.
        For example,


        Unless you're using a specific tool that requires the FASTA+QUAL format, I see no reason to do the conversion. The FASTQ format is the most common file format to represent sequences with their quality scores, and is the standard accepted by nearly all programs.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM
        • seqadmin
          Techniques and Challenges in Conservation Genomics
          by seqadmin



          The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

          Avian Conservation
          Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
          03-08-2024, 10:41 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Yesterday, 06:37 PM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, Yesterday, 06:07 PM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-22-2024, 10:03 AM
        0 responses
        51 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 03-21-2024, 07:32 AM
        0 responses
        67 views
        0 likes
        Last Post seqadmin  
        Working...
        X