Has anyone else been getting PCR products that are exactly double the size of the template? I am seeing this occur with the TruSeq kits and also the KAPA enzyme. I have heard some theories - too much input (seems unlikely), samples clumping together, etc.
I'd love to hear if other people have figured out what is causing this problem. It seems to be a new one over here.
Thanks!
I'd love to hear if other people have figured out what is causing this problem. It seems to be a new one over here.
Thanks!
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