Is anybody doing ChIP Seq using SPRIworks and Illumina's Tru-Seq adapters? I'd be curious to hear what amount of input and adapter is working for folks.
We've been doing ~100 ng of input for WCEs, and 1:200 or possibly even 1:50 adapter. For IPs basically I've been using the whole thing and 1:200 dilution of adapters. But I don't know really know if this is totally optimal and I'd like to hear about other groups and their results.
Thanks in advance.
We've been doing ~100 ng of input for WCEs, and 1:200 or possibly even 1:50 adapter. For IPs basically I've been using the whole thing and 1:200 dilution of adapters. But I don't know really know if this is totally optimal and I'd like to hear about other groups and their results.
Thanks in advance.